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Medico Nepal

Guide for Production of Local Hand Sanitizer

Materials required (small volume production)

REAGENTS FOR FORMULATION 1 (Recommended amounts for 10-litre preparations)

REAGENTS FOR FORMULATION 2:(Recommended amounts for 10-litre preparations)

• Ethanol 96%

 (8333 ml)

• Isopropyl alcohol 99.8% (7515 ml)

• Hydrogen peroxide 3%: used to inactivate contaminating bacterial spores in the solution and is not an active substance for hand antisepsis.

(417 ml)

• Hydrogen peroxide 3%

(417 ml)

• Glycerol 98%: used as humectant, but other emollients may be used for skin care, provided that they are cheap, widely available and miscible in water and alcohol and do not add to toxicity, or promote allergy

(145 ml)

• Glycerol 98%

 (145 ml)

• Sterile distilled or boiled cold water

• Sterile distilled or boiled cold wate


  • 10-litre glass or plastic bottles with screw-threaded stoppers or 50-litre plastic tanks (preferably in polypropylene or high density polyethylene, translucent so as to see the liquid level) or Stainless steel tanks with a capacity of 80–100 litres (for mixing without overflowing)
  • Wooden, plastic or metal paddles for mixing
  • Measuring cylinders and measuring jugs
  • Plastic or metal funnel
  • Plastic or metal funnel
  • 100 ml plastic bottles with leak-proof tops
  • 500 ml glass or plastic bottles with screw tops



  1. The alcohol for the formula to be used is poured into the large bottle or tank up to the graduated mark.
  2. Hydrogen peroxide is added using the measuring cylinder.
  3. Glycerol is added using a measuring cylinder. As glycerol is very viscous and sticks to the wall of the measuring cylinder, it should be rinsed with some sterile distilled or cold boiled water and then emptied into the bottle/tank.
  4. The bottle/tank is then topped up to the 10-litre mark with sterile distilled or cold boiled water.
  5. The lid or the screw cap is placed on the tank/bottle as soon as possible after preparation, in order to prevent evaporation.
  6. The solution is mixed by shaking gently where appropriate or by using a paddle.
  7. Immediately divide up the solution into its final containers (e.g. 500 or 100 ml plastic bottles), and place the bottles in quarantine for 72 hours before use. This allows time for any spores present in the alcohol or the new/re-used bottles to be destroyed.

General Tips:

  • For external use only
  • Avoid contact with eyes
  • Keep out of the reach of children
  • Use: Apply a palm full of alcohol-based hand rub and cover all surfaces of the hands. Rub hands until dry
  • Flammable: keep away from flame and heat
  • Production and storage facilities should ideally be air conditioned or cool rooms. No naked flames or smoking should be permitted in these areas.